Oil Red O Stain Isopropanol Special Stains In Histopathology Laboratory
RamirezZacarias, JL, CastroMunozledo, F & KuriHarcuch, W Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O Histochemistry 97, 493• Oil Red O stock solution (Sigma product #O0625, 150 mg in 50 mL of 100% isopropanol) resources/protocols Differentiation and Staining 1 After the desired number of expansion passages, seed the cells to be differentiated at approximately Prepare the Oil Red O stain solution by mixing 3 parts of the stock solution with 2 parts of de
Oil red o staining protocol sigma
Oil red o staining protocol sigma-Oil Red O for microscopy;Solvent Red 27 CAS Number
Quantification Of Lipid Abundance And Evaluation Of Lipid Distribution In Caenorhabditis Elegans By Nile Red And Oil Red O Staining Protocol
Furthermore, optimization of the imaging settings in order to specifically image intracellular lipid droplets stained with oil red O or Bodipy493/503 is shown When costaining lipid droplets with associated proteins, Bodipy493/503 should be the dye of choice, since oil red O creates precipitates on the lipid droplets blocking the lightProcedure Place frozen sections at RT 30min Preheat Oil Red O Stain to 60°C Place frozen sections in 10% Formalin for 25 minutes then rinse slide in tap water (fixed slide may skip this step) Circle the tissue by Dako Pen Place slide in Propylene glycol for 2 minutes Place slide in Oil Red O Stain heated to 60°C for 6 minutesGlycerol gelatin (Sigma) PROCEDURE 1 Prepare fresh oil redO 2 Fix section in 4% paraformaldehyde/PBS or 10 % formalin for 5 min at room temp Blot 3 60% isopropyl alcohol for 5 min at room temp Blot 4 Stain with filtered oil redO for 10 min at room temp Blot 5 60% isopropyl alcohol for 2 min at room temp Blot 6 Rinse once with dH Blot 7 Stain with
Dark red, sediment free Absorbance 0 050 diluted 025ml to 50 ml with Isopropyl alcohol Lambda Max 510 517 nm Specification PRD0ZQ The current Specification sheet may be available at SigmaAldrichcom For further1 Dilute Oil Red O Stock solution 32 using deionized H 2 O to make Oil Red O working solution Example 3mL Oil Red O stock 2mL deionized H 2 O 2 Place a piece of Whatman paper inside the funnel and filter the Oil Red O working solution Alternatively, a syringe filter unit can be used in place of a Whatman paper/funnel system toArticle Snippet Dimethyl sulfoxide (DMSO), formaldehyde, dexamethasone (DEX), 3isobutyl1methylisobutylxanthine (IBMX), triton X100 and Oil Red O staining powder were purchased from SigmaAldrich (St Louis, MO, USA) and the cell counting kit8 (CCK8) was purchased from Dojindo Laboratories (Kumamoto, Japan)
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After fixation and two further washes in PBS, oil red solution 05% oil red (w/v) (Sigma) in isopropanol was added for 10 min After incubation at room temperature (RT), oil red was removed, and cells were washed twice in PBS and stained with Harris hematoxylin (Surgipath, Bretton, UK) for 45 seconds Subsequently, cells were washed in tapOil Red O staining of adipocytes (12 well plate format) 1 Prepare Oil Red O Stock Solution a Oil Red O Stock Solution Dissolve 035 g Oil Red O (Sigma, Cat# O0625) in 100 ml of 100 %isopropanol Place on the stir plate for minimum of 1hr Filter (04 µM
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