Oil Red O Stain Isopropanol Special Stains In Histopathology Laboratory
RamirezZacarias, JL, CastroMunozledo, F & KuriHarcuch, W Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O Histochemistry 97, 493• Oil Red O stock solution (Sigma product #O0625, 150 mg in 50 mL of 100% isopropanol) resources/protocols Differentiation and Staining 1 After the desired number of expansion passages, seed the cells to be differentiated at approximately Prepare the Oil Red O stain solution by mixing 3 parts of the stock solution with 2 parts of de
Oil red o staining protocol sigma
Oil red o staining protocol sigma-Oil Red O for microscopy;Solvent Red 27 CAS Number
Quantification Of Lipid Abundance And Evaluation Of Lipid Distribution In Caenorhabditis Elegans By Nile Red And Oil Red O Staining Protocol
Furthermore, optimization of the imaging settings in order to specifically image intracellular lipid droplets stained with oil red O or Bodipy493/503 is shown When costaining lipid droplets with associated proteins, Bodipy493/503 should be the dye of choice, since oil red O creates precipitates on the lipid droplets blocking the lightProcedure Place frozen sections at RT 30min Preheat Oil Red O Stain to 60°C Place frozen sections in 10% Formalin for 25 minutes then rinse slide in tap water (fixed slide may skip this step) Circle the tissue by Dako Pen Place slide in Propylene glycol for 2 minutes Place slide in Oil Red O Stain heated to 60°C for 6 minutesGlycerol gelatin (Sigma) PROCEDURE 1 Prepare fresh oil redO 2 Fix section in 4% paraformaldehyde/PBS or 10 % formalin for 5 min at room temp Blot 3 60% isopropyl alcohol for 5 min at room temp Blot 4 Stain with filtered oil redO for 10 min at room temp Blot 5 60% isopropyl alcohol for 2 min at room temp Blot 6 Rinse once with dH Blot 7 Stain with
Dark red, sediment free Absorbance 0 050 diluted 025ml to 50 ml with Isopropyl alcohol Lambda Max 510 517 nm Specification PRD0ZQ The current Specification sheet may be available at SigmaAldrichcom For further1 Dilute Oil Red O Stock solution 32 using deionized H 2 O to make Oil Red O working solution Example 3mL Oil Red O stock 2mL deionized H 2 O 2 Place a piece of Whatman paper inside the funnel and filter the Oil Red O working solution Alternatively, a syringe filter unit can be used in place of a Whatman paper/funnel system toArticle Snippet Dimethyl sulfoxide (DMSO), formaldehyde, dexamethasone (DEX), 3isobutyl1methylisobutylxanthine (IBMX), triton X100 and Oil Red O staining powder were purchased from SigmaAldrich (St Louis, MO, USA) and the cell counting kit8 (CCK8) was purchased from Dojindo Laboratories (Kumamoto, Japan)
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